Analytical Sciences

Abstract − Analytical Sciences, 21(11), 1303 (2005).

Physicochemical Characterization of the Strychnos Alkaloids by Capillary Zone Electrophoresis
Shaoping FU, Feng ZHANG, Xiaozhe ZHANG, Qing XU, Hongbin XIAO, and Xinmiao LIANG
Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, P. R. China
A capillary zone electrophoresis (CZE) method has been developed for investigating the physicochemical characteristics of five Strychnos alkaloids in Strychnos nux-vomica L. Firstly, the dissociation constants of the five Strychnos alkaloids were determined, based on the relation between the effective mobility of the solutes and the buffer pH. The mathematical relationship was strictly deduced from the fundamental electrophoretic theory and the dissociation equilibrium. Secondly, an equation describing the relation between the migration time of alkaloids of similar structure and their molecular weights was developed and used to predict the migration order and to calculate the electrosomotic velocity. The results predicted by the theory agreed with those from experiments.