Analytical Sciences

Abstract − Analytical Sciences, 21(11), 1291 (2005).

Determination of Pesticide Residues by GC-MS Using Analyte Protectants to Counteract the Matrix Effect
Consuelo SÁNCHEZ-BRUNETE, Beatriz ALBERO, Germán MARTÍN, and José L. TADEO
Departamento de Medio Ambiente, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Carretera de la Coruña Km 7, 28040 Madrid, Spain
An analytical method was developed to determine pesticides of various chemical classes in soil, juice and honey using analyte protectants to counteract the enhancement of the chromatographic response produced by the presence of matrix components (matrix effect). This effect was more pronounced for soil and honey samples than for juice samples; regarding the pesticide chemical class, organochlorine pesticides were less affected by the presence of matrix components than triazines and organophosphorus pesticides. Several analyte protectants (2,3-butanediol, L-gulonic acid γ-lactone, corn oil and olive oil) were tested for counteracting the observed matrix effect. L-Gulonic acid γ-lactone was an effective protecting agent for most of the pesticides studied in soil and honey samples, whereas olive oil was very effective for juice samples. The combination of these two protectants was found to be an effective analyte protectant for all compounds in soil and honey samples.