Analytical Sciences

Abstract − Analytical Sciences, 20(9), 1265 (2004).

Silica-hemin Composite Nanoparticles as New Biocatalyst to Highly Sensitive Determination of Glucose in Human Serum
Wei YANG, Hong ZHENG, Wen-Tao YUAN, and Jin-Gou XU
The Key Laboratory of Analytical Science of MOE, Department of Chemistry, Xiamen University, Xiamen 361005, P. R. China
The preparation and utilization of a novel composite silica-hemin nanoparticles (CSHNs) as mimetic peroxidase are reported in this article. Experimental results showed that the composite nanoparticles had unique advantages over free hemin molecules in good stability and highly catalytic activity. By employing these nanoparticles as biocatalyst, we developed a new spectrofluorometric method for the determination of trace level glucose. The calibration graph for glucose was linear over the range 4.0 × 10-7 mol/L - 7.0 × 10-5 mol/L, with a detection limit of 1.0 × 10-7 mol/L. The proposed method has been successfully applied to the determination of glucose in serum samples and offers the advantages of being rapid, stable, sensitive, and renewable.