Analytical Sciences

Abstract − Analytical Sciences, 20(9), 1247 (2004).

Sugar-Sensitive Thin Films Composed of Concanavalin A and Glycogen
Katsuhiko SATO, Yoko IMOTO, Jun SUGAMA, Shunsuke SEKI, Hiroyuki INOUE, Tsuyoshi ODAGIRI, and Jun-ichi ANZAI
Graduate School of Pharmaceutical Sciences, Tohoku University, Aramaki, Aoba-ku, Sendai 980-8578, Japan
Sugar-sensitive thin films were prepared by a layer-by-layer deposition of concanavalin A (Con A) and glycogen on the surface of a quartz slide and their sugar-induced decomposition was studied. The Con A/glycogen multilayer films can be decomposed by exposing them to sugar solutions (D-glucose, D-mannose, methyl- α-D-glucose and methyl- α-D-mannose), as a result of displacement of sugar residues of glycogen from the binding sites of Con A by the free sugar added in the solution. The rate of decomposition significantly depended on the type of sugar and its concentration.