Analytical Sciences

Abstract − Analytical Sciences, 20(4), 707 (2004).

Spectrofluorometric Determination of Hydrogen Peroxide Based on Oxidative Catalytic Reactions of p-Hydroxyphenyl Derivatives with Metal Complexes of Thiacalix[4]arenetetrasulfonate on a Modified Anion-Exchanger
Junichi ODO, Kozue MATSUMOTO (née MIEDA), Eri SHINMOTO, Yoko HATAE, and Atsushi SHIOZAKI
Department of Biological Chemistry, Faculty of Science, Okayama University of Science, Ridai-cho, Okayama 700-0005, Japan
The peroxidase-like catalytic activity of metal complexes of thiacalix[4]arenetetrasulfonate (TCAS[4]) on a modified anion-exchanger (Men+-TCAS[4]A-500; Men+ = H2, Fe3+, Fe2+, Mn3+, Co3+, Co2+, Cu2+, Zn2+, Ni2+) for the oxidation of p-hydroxyphenyl derivatives to produce fluorescent substances in the presence of hydrogen peroxide has been investigated. Among the Men+-TCAS[4]A-500 tested, Fe3+-TCAS[4]A-500 exhibited the highest level of catalytic activity for the oxidation of p-acetoamidophenol in a carbonate buffer solution of pH 10. The catalytic activity of Fe3+-TCAS[4]A-500 was then used for the spectrofluorometric determination of hydrogen peroxide. The calibration curve for the Fe3+-TCAS[4]A-500 method was linear over a range spanning from 0.1 to 5.0 µg of hydrogen peroxide in a 1.0 ml sample solution.