Analytical Sciences

Abstract − Analytical Sciences, 20(4), 585 (2004).

An Anti E. Coli O157:H7 Antibody-Immobilized Microcantilever for the Detection of Escherichia Coli (E. coli)
Jing ZHANG and Hai-Feng JI
Department of Chemistry and the Institute for Micromanufacturing, Louisiana Tech University, Ruston, Louisiana 71272, USA
A silicon microcantilever sensor was developed for the detection of Escherichia coli O157:H7. The microcantilever was modified by anti-E. coli O157:H7 antibodies on the silicon surface of the cantilever. When the aquaria E. coli O157:H7 positive sample is injected into the fluid cell where the microcantilever is held, the microcantilever bends upon the recognition of the E. coli O157:H7 antigen by the antibodies on the surface of the microcantilever. A negative control sample that does not contain E. coli O157:H7 antigen did not cause any bending of the microcantilever. The detection limit of the sensor was 1 × 106 cfu/mL when the assay time was < 2 h.