Analytical Sciences

Abstract − Analytical Sciences, 20(11), 1489 (2004).

Measurement of Circular Dichroism Spectra of Liquid/Liquid Interface by Centrifugal Liquid Membrane Method
Sayaka WADA,* Kazuhiko FUJIWARA,** Hideaki MONJUSHIRO,* and Hitoshi WATARAI*
*Department of Chemistry, Graduate School of Science, Osaka University, 1-1, Machikaneyama-cho, Toyonaka, Osaka 560-0043, Japan
**Faculty of Engineering and Resource Science, Akita University, 1-1, Tegatagakuen-cho, Akita 010-8502, Japan
The direct measurement of the circular dichroism (CD) spectra of liquid/liquid interface has been achieved for the first time by the centrifugal liquid membrane (CLM) method combined with a conventional CD spectropolarimetry. In the sample chamber of the CD spectropolarimeter, a cylindrical glass cell containing small amounts of organic and aqueous phases was rotated at ca. 7000 rpm to generate a two-phase liquid membrane with a high specific interfacial area. The CD spectra of the J-aggregate of protonated 5,10,15,20-tetraphenylporphyrin formed at the toluene/sulfuric acid interface in the rotating cell have been measured. The results demonstrated the novelty and advantages of this method.