Analytical Sciences


Abstract − Analytical Sciences, 19(7), 1013 (2003).

Differentiation of Species of theBaccharis Genus by HPLC and Chemometric Methods
Audrey A. S. G. LONNI, Ieda S.SCARMINIO,  Lucas M. C. SILVA, and Dalva T.FERREIRA
Departamento de Química,Universidade Estadual de Londrina, Caixa Postal 6001, CEP 86051-970,Londrina, Paraná, Brazil
Phytochemical investigation of the aerialparts of three Baccharis species (Asteraceae family) was performedusing HPLC and chemometric methods, with the objective of distinguishingbetween three morphologically very similar species: Baccharisgenistelloides Persoon var. trimera (Less.) DC, B.milleflora (Less.) DC and B. articulata (Lam.) Persoon. Withthe help of Principal Component Analysis (PCA) and variance weights, it waspossible to characterize the chromatographic profiles of the alcoholicextracts of the three species. Application of Soft Independent Modeling ofClass Analogy (SIMCA) and K-Nearest Neighbor (KNN) methods on a trainingset of 74 extracts resulted in models that correctly classified all eightsamples in an independent test set.