Analytical Sciences

Abstract − Analytical Sciences, 19(5), 767 (2003).

Electroanalytical Properties of Aldehyde Biosensors with a Hybrid-Membrane Composed of an Enzyme Film and a Redox Os-Polymer Film
Toshio YAO  and Shotaro HANDA
Department of Applied Chemistry, Graduate School of Engineering, Osaka Prefecture University, 1-1 Gakuencho, Sakai, Osaka 599-8531, Japan
Aldehyde biosensors were constructed by cross-linking formaldehyde dehydrogenase (FDH) or aldehyde dehydrogenase (ADH) and bovine serum albumin on the surface of a redox Os-polymer-coated electrode. The prepared aldehyde biosensors responded rapidly (within 30 s) to aldehydes without the addition of a soluble mediator, because the inner redox Os-polymer film effectively mediated the electron transfer from NADH generated enzymatically into the outer enzyme film to a glassy carbon electrode. An FDH/Os-polymer electrode responded linearly over the concentration range of 2 x 10-6 - 5 x 10-4 M for formaldehyde, while an ADH/Os-polymer electrode, though responding similarly to long chain aldehydes, such as propionaldehyde and butylaldehyde, responded linearly over the concentration range of 4 x 10-6 - 2 x 10-4 M for acetaldehyde.