Analytical Sciences

Abstract − Analytical Sciences, 19(4), 633 (2003).

A Miniaturized Fluorescence Detector with a Windowless Flow Cell Using a Light-Emitting Diode
Bingcheng YANG and Yafeng GUAN 
Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 116012, P. R. China
A miniaturized fluorescence detector using a high-brightness light-emitting diode as an excitation source was constructed and evaluated. A windowless flow cell based on a commercial four-port cross fitting was designed to reduce the straylight level and to eliminate the optical alignment. The observed detection limit for fluorescein was 26 nM in the continuous-flow mode. The error in the reproducibility of the responses was evaluated by the FIA method, and was found to be within 2% RSD.