Analytical Sciences

Abstract − Analytical Sciences, 19(4), 621 (2003).

Determination of Thiobarbituric Acid Adduct of Malondialdehyde Using On-line Microdialysis Coupled with High-Performance Liquid Chromatography
Jenn-Yuan SHEU,* Hsiao-Pei KU,* Wei-Chang TSENG,* Min-Tzu CHEN,* Li-Yu TSAI,** and Yeou-Lih HUANG** 
*Graduate Institute of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan
**School of Technology for Medical Sciences, Kaohsiung Medical University, Kaohsiung, Taiwan
An on-line analytical system for the continuous monitoring of malondialdehyde (MDA) was developed. This method involves the use of microdialysis perfusion, on-line derivatization and on-line HPLC analysis. This method gave a linear response for MDA concentrations and HPLC peak areas in the range from 0.051 µM to 2.43 µM. The intra-day (RSD = 1.6 - 10.5%) and inter-day (RSD = 1.1 - 9.3%) precisions were acceptable. The average in vitro probe recovery of MDA standard was 18.4 ± 1.0%. The detection limit was 0.03 µM, corresponding to 0.6 pmol for an injection volume of 20 µl. This method was used for in vitro peroxidation investigations, which provided evidence for elevated MDA levels following the incubation of metal ions to a linoleic acid solution.