Analytical Sciences

Abstract − Analytical Sciences, 19(4), 579 (2003).

Sensitive and Simple Cloud-point Preconcentration Atomic Absorption Spectrometry: Application to the Determination of Cobalt in Urine Samples
Jamshid L. MANZOORI  and Ghasem KARIM-NEZHAD
Department of Analytical Chemistry, Faculty of Chemistry, The University of Tabriz, Tabriz, Iran
A new approach for developing a cloud-point extraction-flame atomic absorption spectrometric method has been described and used for the determination of cobalt. In this approach, water was removed from the final diluted surfactant rich phase obtained in cloud-point extraction procedure. The results indicated that removing water from this phase increased the enhancement factor by 4-fold. 1-(2-Pyridylazo)-2-naphthol (PAN) and octylphenoxypolyethoxyethanol (Triton X-114) were used as a hydrophobic ligand and a nonionic surfactant, respectively. The chemical variables affecting the preconcentration step were optimized. The effect of the water concentration in the final diluted methanolic surfactant solution on the analytical signal was investigated. The results showed that the analytical signal decreased by 30% and 52% in 15% and 25% water concentrations in methanol, respectively. An enhancement factor of 115 was obtained for cobalt extracted from only 10 ml of a sample. The detection limit obtained under the optimal condition was 0.38 µg l-1. The proposed procedure was applied to the determination of cobalt in urine samples.