Analytical Sciences

Abstract − Analytical Sciences, 19(11), 1537 (2003).

Enhanced Peroxidase Activity of Hemoglobin in a DNA Membrane and Its Application to an Unmediated Hydrogen Peroxide Biosensor
Libin SHANG, Ziyi SUN, Xingwu WANG, and Genxi LI 
Department of Biochemistry and National Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210093, P. R. China
Hemoglobin can exhibit not only a direct electron transfer reacting after being entrapped in a DNA membrane, but also a greatly enhanced peroxidase activity toward the reduction of hydrogen peroxide. Based on the direct electrochemical property and nice enzymatic activity of the protein in a DNA membrane, a reagentless hydrogen peroxide biosensor was prepared. The peak current related to hydrogen peroxide was linearly proportional to its concentration in the range of 1.9 x 10-6 - 6.8 x 10-4 mol L-1. The detection limit was 1 x 10-6 mol/L.