Analytical Sciences

Resonance Rayleigh-Scattering Method for the Determination of Vitamin B₁ with Methyl Orange

Shaopu LIU,  Zhuyuan ZHANG, Hongqun LUO, and Ling KONG

Institute of Environmental Chemistry, Southwest China Normal University, Chongqing, 400715, China

In a pH 2.3 - 3.0 acid medium, the resonance Rayleigh scattering (RRS) intensity is greatly enhanced when vitamin B₁ reacts with Methyl Orange to form an ion-association complex. The maximum RRS peak appears at 588 nm, another higher peak is at 403 nm and there are two smaller RRS peaks at 800 nm and 288 nm. The RRS intensity is directly proportional to the concentration of vitamin B₁ in the range of 0 - 400 ng ml^-1. The method has high sensitivity and the detection limit (3sigma) for vitamin B₁ is 7.2 ng ml^-1. The effects of coexisting substances on the determination of vitamin B₁ were investigated, and the results show that this method has good selectivity and can be applied to the direct determination of vitamin B₁ in composite vitamin B tablets and multivitamin tablet samples.

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