Analytical Sciences

Abstract − Analytical Sciences, 18(6), 645 (2002).

Spectroelectrochemistry Study on the Electrochemical Reduction of Ethidium Bromide
Xinhui HU, Qingjiang WANG, Pingang HE, and Yuzhi FANG 
Department of Chemistry, East China Normal University, Shanghai 200062, China
The electrochemical reduction mechanism of ethidium bromide was first studied by spectroelectrochemistry. This reduction was proved to be a two-step process by cyclic voltammetry, differential pulse voltammetry and spectroelectrochemistry, in which each step was proved to be a one-electron transfer process by a spectropotentiostatic fluorescence technique. Hydroethidine was confirmed to be the final product by comparing the spectrum of the product of the electrochemical reduction to that of the product of the chemical reduction of ethidium bromide, and a carbon-centered radical was concluded to be a reasonable intermediate product during the electrochemical reduction of ethidium bromide.