Analytical Sciences

Abstract − Analytical Sciences, 18(2), 161 (2002).

Syntheses and Characterizations of4-(3,17 beta-Dihydroxyestra-1,3,5(10)-trien-6alpha- and6beta-yl)amino-7-nitro-2,1,3-benzoxadiazoles as FluorescentProbes
Tomoyuki OE,  Miki IKUTA,and Toshimasa TOYO'OKA
Department of Analytical Chemistry,School of Pharmaceutical Sciences, University of Shizuoka, 52-1 Yada,Shizuoka 422-8526, Japan
4-(3,17beta-Dihydroxyestra-1,3,5(10)-trien-6alpha- and6beta-yl)amino-7-nitro-2,1,3-benzoxadiazoles have been synthesized andcharacterized as fluorescent probes for use in a receptor assay and/or ahomogeneous immunoassay for estradiol. The fluorescence intensities arestrongly dependent upon the solvent polarity used. The intensities inwater were reduced to less than 1% of those in ethyl acetate, and a blueshift was also observed in polar solvents. The quenched fluorescence inaqueous solution was recovered by adding bovine serum albumin or ananti-estradiol antibody. Adding intact estradiol inhibited thefluorescence recovered by the antibody.