Analytical Sciences

Abstract − Analytical Sciences, 18(2), 147 (2002).

A Novel Two-Enzyme AmperometricElectrode for Lactose Determination
Handan GULCE,* Ahmet GULCE,* and Attila YILDIZ**
*Department of Chemistry, SuleymanDemirel University, Isparta, 32260, Turkey
**Department of Chemistry,Hacettepe University, Beytepe, Ankara, 06532, Turkey
Coimmobilization of beta-galactosidase andglucose oxidase in a redox polymer, polyvinylferrocenium perchlorate(PVF+ClO4-), led to the development of anenzyme electrode for the determination of lactose. The amperometricresponse of the electrode was measured at +0.70 V vs. SCE, whichwas due to the electrooxidation of enzymatically producedH2O2. The effects of the substrate and bufferconcentrations as well as the pH on the electrode response wereelucidated.