Abstract − Analytical Sciences, 18(10), 1117 (2002).
Determination of Histamine by Capillary Zone Electrophoresis with Amperometric Detection
Liyao ZHANG, Weihua HUANG, Zongli WANG, and Jieke CHENG
Department of Chemistry, Wuhan University, Wuhan 430072, China
Capillary zone electrophoresis was employed for the determination of histamine using end-column amperometric detection with a carbon fiber microelectrode, at a constant potential. The optimum conditions of separation and detection were 10 mmol/L phosphate buffer, pH 5.6 for the buffer solution, 15 kV for the separation voltage, and 1.35 V (versus SCE) for the detection potential. The linear range was from 6.3 x 10-7 to 1.5 x 10-5 mol/L with the regression coefficient of 0.9997, and the detection limit was 4.0 x 10-7 mol/L (S/N = 3). The proposed method was successfully applied to the direct determination of histamine in the beer samples without any sample clean-up procedures.
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