Analytical Sciences

Identification of Luminescent Europium beta-Diketonates Using High-Resolution Emission Spectrometry and Direct-Inlet Electron Ionization Mass Spectrometry

Shoji KURATA* and Masatoshi NAGAI**

*Criminal Investigation Laboratory, Metropolitan Police Department, Chiyoda, Tokyo 100-8929, Japan
**Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology, Koganei, Tokyo 184-8588, Japan

The high-resolution emission spectra measured using a microscope Raman spectrometer and direct-inlet electron ionization mass spectrometry (DI-EIMS) were studied for the rapid analysis of a trace amount of various europium beta-diketonates in order to utilize them as fluorescent markers. Five kinds of europium beta-diketonates were distinguished from the emission spectra obtained using the Raman spectrometer. For DI-EIMS, the electron ionization mass spectra of the europium beta-diketonates showed the characteristic fragment ion peaks due to the isotope effect of ¹⁵¹Eu and ¹⁵³Eu. The DI-EIMS was very useful for identifying the compounds compared with spectrofluorometry. The scan and selected ion-monitoring modes of DI-EIMS showed the detection limits of the samples at levels of hundreds and tens of nanograms (ng), respectively.

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