Analytical Sciences

Abstract − Analytical Sciences, 17(9), 1079 (2001).

Identification of Luminescent Europium beta-Diketonates Using High-Resolution Emission Spectrometry and Direct-Inlet Electron Ionization Mass Spectrometry
Shoji KURATA* and Masatoshi NAGAI**
*Criminal Investigation Laboratory, Metropolitan Police Department, Chiyoda, Tokyo 100-8929, Japan
**Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology, Koganei, Tokyo 184-8588, Japan
The high-resolution emission spectra measured using a microscope Raman spectrometer and direct-inlet electron ionization mass spectrometry (DI-EIMS) were studied for the rapid analysis of a trace amount of various europium beta-diketonates in order to utilize them as fluorescent markers. Five kinds of europium beta-diketonates were distinguished from the emission spectra obtained using the Raman spectrometer. For DI-EIMS, the electron ionization mass spectra of the europium beta-diketonates showed the characteristic fragment ion peaks due to the isotope effect of 151Eu and 153Eu. The DI-EIMS was very useful for identifying the compounds compared with spectrofluorometry. The scan and selected ion-monitoring modes of DI-EIMS showed the detection limits of the samples at levels of hundreds and tens of nanograms (ng), respectively.