Analytical Sciences

Chemiluminescence Flow Biosensor System for Cholesterol with Immobilized Reagents

Yuming HUANG*, Chao ZHANG** and Zhujun ZHANG**^, *** *

*Department of Chemistry, Nanjing University, Nanjing 210008, P. R. China
**Department of Chemistry, Shaanxi Normal University, Xi'an 710062, P. R. China
***Department of Chemistry, Southwest Normal University, Beibei, Chongqing 400715, P. R. China

A chemiluminescence biosensor combined with flow injection analysis has been developed for determining cholesterol. Cholesterol oxidase was immobilized onto amine-modified silica gel via glutaraldehyde activation and packed in a column. The analytical reagents, including luminol and ferricyanide, were electrostatically coimmobilized on an anion-exchange column. Cholesterol was sensed by the CL reaction between hydrogen peroxide released from the enzymatic reaction and luminol and ferricyanide, which were released from a column with immobilized reagents by elution. The calibration graph was linear over the range 5x10^-6 to 1x10^-4 g ml^-1 and the detection limit was 5x10^-6 g ml^-1. The proposed method has been successfully applied to the determination of cholesterol in human serum. (Keywords: Immobilized reagent, chemiluminescence, flow sensor, cholesterol)

J-STAGE: View this article in J-STAGE