Analytical Sciences


Abstract − Analytical Sciences, 15(12), 1257 (1999).

Batch-Type Detection Cell Using a Peroxyoxalate Chemiluminescence System for Capillary Electrophoresis
Kazuhiko TSUKAGOSHI +, Miwa OTSUKA, Masahiko HASHIMOTO, Riichiro NAKAJIMA and Kazuo KONDO
Department of Chemical Engineering and Materials Science, Faculty of Engineering, Doshisha University, Kyotanabe, Kyoto 610-0321, Japan
A batch-type chemiluminescence detection cell using a peroxyoxalate system was easily combined with capillary electrophoresis equipment; the device was operated without any tedious procedures. Peroxyoxalate reagents and hydrogen peroxide were examined in detail concerning their usage and concentration with the cell. Fluorescence-labeled a-amino acids showed the detection limits of 10 - 100 nM order (S/N=3), which were approximately 2 or 3 orders of magnitude lower than those obtained with the absorbance. A mixture sample of the labeled a-amino acids was successfully separated and detected. (Keywords: Capillary electrophoresis, chemiluminescence, peroxyoxalate, optical fiber, batch-type, a-amino acid)