Analytical Sciences

Abstract − Analytical Sciences, 14(3), 519 (1998).

Flow Analysis of Hydrogen Peroxide with a Dye-Formation Reaction Catalyzed by an Ion-Exchange Resin Modified with Mn 3+ -Tetrakis(sulfophenyl)porphine
Masaki MIFUNE*, Takashi MUKUNO**, Misato TANI*, Akimasa IWADO**, Junichi ODO*, Noriko MOTOHASHI*** and Yutaka SAITO* *
*Faculty of Pharmaceutical Sciences, Okayama University, Tsushima-Naka, Okayama 700, Japan
**The Graduate School of Natural Science and Technology, Okayama University, Tsushima-Naka, Okayama 700, Japan
***Kobe Pharmaceutical University, Motoyamakita, Higashinada, Kobe 658, Japan
The present flow analysis of hydrogen peroxide is based on a measurement of the absorbance of quinoid-dye formed by a following reaction (1) using an anion-exchange resin modified with manganese(III)-tetrakis(sulfophenyl)porphine (Mn-TSPPr), which catalyzes the reaction (1), just like an immobilized peroxidase: 4-aminoantipyrine+4-chlorophenol+2H2O2 quinoid dye+4H2O [reaction (1)]. Mn-TSPPr is more advantageous than immobilized peroxidase in terms of storage and stability for both temperature and moisture. The present facilitated method is satisfactory in reproducibility and sensitivity. (Keywords: Flow injection analysis, hydrogen peroxide, anion-exchange resin modified with manganese(III) tetrakis(sul-fophenyl) porphine, 4-aminoantipyrine, 4-chlorophenol)