Analytical Sciences

Analysis of Charged Cyclodextrin Derivatives by On-Line Capillary Electrophoresis-Ionspray-Mass Spectrometry

Yoshihide TANAKA* * , Yasuhiro KISHIMOTO* and Shigeru TERABE**

*Department of Analytical Chemistry, Nippon Boehringer Ingelheim Co. Ltd., 3-10-1, Yato, Kawanishi, Hyogo 666-01, Japan
**Faculty of Science, Himeji Institute of Technology, Kamigori, Hyogo 678-12, Japan

On-line capillary electrophoresis-ionspray-mass spectrometry (CE-ISP-MS) was applied to a composition analysis of charged cyclodextrin (CD) derivatives. Quaternary ammonium b-CD (QA- b-CD) and carboxymethylated g-CD (CM-g-CD), which were used as chiral selectors for enantiomer separations by CE, were analyzed. As for QA- b-CD, eight com-ponents having different degrees of the quaternary ammonium substitution could be identified based on their mass spec-tra. The components were detected as either molecular ions or fragment ions. On the other hand, six components of CM-g-CD were detected as bivalent molecular ions. Such composition analyses were difficult using CE with only the UV detector. CE-MS was found to be a promising technique for a composition analysis of mixed compounds. (Keywords: Capillary electrophoresis-mass spectrometry, ionspray interface, composition analysis, charged cyclodextrin)

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