Analytical Sciences

Abstract − Analytical Sciences, 37(8), 1139 (2021).

Oxide Nanowire Microfluidic Devices for Capturing Single-stranded DNAs
Marina MUSA,*1 Takao YASUI,*1,*2,*3 Zetao ZHU,*1 Kazuki NAGASHIMA,*2,*4 Miki ONO,*1 Quanli LIU,*1 Hiromi TAKAHASHI,*1 Taisuke SHIMADA,*1 Akihide ARIMA,*1 Takeshi YANAGIDA,*4,*5 and Yoshinobu BABA*1,*3,*6
*1 Department of Biomolecular Engineering, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa, Nagoya 464-8603, Japan
*2 Japan Science and Technology Agency (JST), Precursory Research for Embryonic Science and Technology (PRESTO), 4-1-8 Honcho, Kawaguchi, Saitama 332-0012, Japan
*3 Institute of Nano-Life-Systems, Institutes of Innovation for Future Society, Nagoya University, Furo-cho, Chikusa, Nagoya 464-8603, Japan
*4 Department of Applied Chemistry, Graduate School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo, Tokyo 113-8656, Japan
*5 Institute of Materials Chemistry and Engineering, Kyushu University, 6-1 Kasuga-Koen, Kasuga, Fukuoka 816-8580, Japan
*6 Institute of Quantum Life Science, National Institutes for Quantum and Radiological Science and Technology, 4-9-1 Anagawa, Inage, Chiba 263-8555, Japan
Since DNA analysis is the fundamental process for most applications in biomedical fields, capturing DNAs with high efficiency is important. Here, we used several oxide nanowire microfluidic devices to capture CpG-rich single-stranded DNAs (ssDNAs) in different pH solutions. All the oxide nanowires exhibited the highest capture efficiency around pH 7 with good capture efficiency shown by each metal oxide; ZnO/ZnO core/shell NWs (71.6%), ZnO/Al2O3 core/shell NWs (86.3%) and ZnO/SiO2 core/shell NWs (86.7%). ZnO/Al2O3 core/shell NWs showed the best performance for capturing ssDNAs under varying pH, which suggests its suitability for application in diverse biological fluids. The capturing efficiencies were attributed to the interactions from phosphate backbones and nucleobases of ssDNAs to each nanowire surface. This finding provides a useful platform for highly efficient capture of the target ssDNAs, and these results can be extended for future studies of cancer-related genes in complex biological fluids.