Analytical Sciences

Abstract − Analytical Sciences, 37(8), 1081 (2021).

A Novel Coumarin-based Fluorescent Probe for Recognition of Copper(II) Ions and Its Application in Bioimaging
Yang SUN, Minghao LI, Yang JIAO, and Chunying DUAN
State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian 116024, China
Simple, accurate and real-time analytical methods are required for the detection of metal ions in a complex environment. In the present work, a fluorescent probe CPB based on coumarin was designed for recognizing Cu2+ ions. The fluorescence of CPB gradually quenched with increasing concentration of Cu2+ ions, due to the interactions between CPB and Cu2+ ions. With the addition of Cu2+ ions, the emission changes of CPB exhibited a good liner relationship toward the Cu2+ ions content in solution. Additionally, CPB could highly selective recognize Cu2+ ions among other metal ions in solution. Bearing the selectivity and fluorescence property toward Cu2+ ions, CPB was successfully applied to monitoring Cu2+ ions in Hela cells and zebrafish.