Analytical Sciences

Abstract − Analytical Sciences, 37(7), 1023 (2021).

Peroxidase-mimicking Activity of PCN-222(Fe) for Colorimetric Sensing of Acetylcholinesterase Activity and Inhibition
Mengfan XIA, Tongtong LIU, and Yaodong ZHANG
Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Changan West Road 620, Xi’an, 710119, China
A colorimetric method for detection of acetylcholinesterase (AChE) activity and inhibition was developed using metal organic frameworks (i.e., PCN-222(Fe)) with peroxidase-like activity. The blue tetramethylbenzidine oxidized by PCN-222(Fe) fades due to the reduction by acetylthiocholine chloride produced from AChE catalysis. The detection method shows a linear range of 0.05 – 10 mU mL−1 and a detection limit of 0.03 mU mL−1 AChE. Average recoveries in serum samples varied from 93 to 115% and relative standard deviation (RSD) was lower than 4.9%.