Analytical Sciences

Abstract − Analytical Sciences, 37(10), 1349 (2021).

Study on the Biosensor Based on Biomimetic PDA Vesicles Fluorescence Resonance Energy Transfer for the Determination of Ovarian Cancer Marker miRNA-21
Yang SUN,*1 Yusheng YAO,*2 Bingrong WANG,*1 Yiying LI,*1 Siming LI,*1 Yuxia SUI,*3 and Bin QIU*4
*1 Department of Gynecology, Fujian Cancer Hospital, Affiliated Cancer Hospital of Fujian Medical University, Fuzhou, Fujian 350014, PR China
*2 Department of Anesthesiology, Shengli Clinical Medical College of Fujian Medical University &Fujian Provincial Hospital, Fuzhou, Fujian 350001, PR China
*3 Department of Pharmacy, Shengli Clinical Medical College of Fujian Medical University & Fujian Provincial Hospital, Fuzhou, Fujian 350001, PR China
*4 Ministry of Education Key Laboratory for Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Fuzhou University, Fuzhou, Fujian 350108, PR China
In recent years, more and more research is being conducted on microRNAs and their involvement in the regulation of autophagy phagocytosis which is closely related to tumor growth. MicroRNA-21 is a kind of small RNA that can regulate gene expression and plays a significant role in autophagy of tumor cells. But the detection of microRNAs had always been a problem in the field of biological analysis. In this study, we designed a new fluorescent sensor for the detection of miRNA-21. The sensor was based on the successful signal reporting by E36-encapsulated vesicles and the specific interaction between E36 and miRNA-21. In the presence of miRNA, the E36/miRNA-21 complex formed and served as a donor molecule inside the acceptor PDA vesicles to amplify the fluorescence through FRET. Additionally, the sensor was applied to detect miRNA-21 in complex biological samples with satisfactory results.