Analytical Sciences

Capillary Electrophoretic Characterization of Water-soluble Carbon Nanodots Formed from Glutamic Acid and Boric Acid under Microwave Irradiation

Toshio TAKAYANAGI,* Sota IWASAKI,** Yuta BECCHAKU,** Shun YABE,*** Kotaro MORITA,*** Hitoshi MIZUGUCHI,* and Naoki HIRAYAMA***

*Graduate School of Technology, Industrial and Social Sciences, Tokushima University, 2-1 Minamijyousanjima-cho, Tokushima 770-8506, Japan
**Graduate School of Advanced Technology and Science, Tokushima University, 2-1 Minamijyousanjima-cho, Tokushima 770-8506, Japan
***Department of Chemistry, Faculty of Science, Toho University, 2-2-1 Miyama, Funabashi 274-8510, Japan

Water-soluble carbon nanodots (CND) were synthesized under microwave irradiation from glutamic acid or glutamic acid–boric acid mixture. The CNDs were collected in an aqueous solution through size fractionation by centrifugal filtration. The CNDs thus prepared were subjected to characterization by capillary electrophoresis (CE). A peak signal of anionic substance was detected in the electropherogram, and it was found to be a major component of the CNDs. The effective electrophoretic mobility of the major component was almost identical over the pH range between 6.7 and 11.6, suggesting that the functional group of amine or boric acid moiety was not included in the CNDs. The effective electrophoretic mobility decreased at an acidic pH of less than 5, and it was suggested that carboxylate moiety was included in the CNDs. A signal of less-charged CNDs was also detected in the electropherogram, and the CNDs were characterized by a CE format of micellar electrokinetic chromatography. Two or four peaks were detected just after the electroosmotic flow; the less-charged CNDs were thus hydrophilic. The affinity interaction was also examined between the major anionic CNDs and a hydrophobic pairing cation. The peak signal of the major anionic CNDs broadened, and its theoretical number of plates decreased in the presence of tetrabutylammonium ion in the separation buffer. A small portion of the anionic CNDs were a little hydrophobic at different degrees, and their effective electrophoretic mobility decreased by the hydrophobic interaction, resulting in peak broadening of the anionic CNDs.

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