Analytical Sciences

Abstract − Analytical Sciences, 36(7), 881 (2020).

Identification of Binding Epitopes of a Fluorinated Compound Bound to Proteins Using 1H and 19F NMR Spectroscopy
Kazuo FURIHATA* and Mitsuru TASHIRO**
*Division of Agriculture and Agricultural Life Sciences, The University of Tokyo, Yayoi, Bunkyo, Tokyo 113-8657, Japan
**Department of Chemistry, College of Science and Technology, Meisei University, Hino, Tokyo 191-8506, Japan
1H/19F NMR-based screening methods were applied to a human serum albumin-fleroxacin complex. Fleroxacin contains three fluorine atoms in a molecule, which is suitable as a model fluorinated compound for NMR analysis with 1H and 19F detection. The 19F{1H} and 1H{1H} saturation transfer difference spectra were acquired and the 1H/19F spin-lattice relaxation rates were measured with and without any selective irradiation of protein resonance to identify the binding epitopes of fleroxacin. Because several 1H signals of fleroxacin resonated close to water, its precise signal intensities were unavailable. The 19F NMR-based screening methods successfully provide complementary information, indicating its importance in the analysis of fluorinated compounds.