Analytical Sciences

Abstract − Analytical Sciences, 36(11), 1345 (2020).

A Sensitive, Simple and Direct Determination of Pantoprazole Based on a “Turn off-on” Fluorescence Nanosensor by Using Terbium-1,10-phenanthroline-silver Nanoparticles
Masoomeh SHAGHAGHI,* Samaneh RASHTBARI,** Asma ABDOLLAHI,* Gholamreza DEHGHAN,** and Abolghasem JOUYBAN***
*Department of Chemistry, Payame Noor University, P. O. Box 19395-3697 Tehran, Iran
**Department of Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran
***Pharmaceutical Analysis Research Center and Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, IranandDigestive Diseases Research Institute, Tehran University of Medical Sciences, Tehran, Iran
A new sensitive, simple, rapid, reliable and selective fluorometric method for the determination of pantoprazole (PAN) in human plasma and a pharmaceutical formulation has been developed. This technique is based on a quenching effect of silver nanoparticles (AgNPs) on the emission intensity of a fluorescent probe, terbium(III)-1,10-phenantroline (Tb(III)-phen) complex (due to a fluorescence resonance energy transfer (FRET) phenomenon between the Tb(III)-phen complex and AgNPs), and then restoring the fluorescence intensity of the Tb(III)-phen-AgNPs system upon the addition of PAN (turn off-on process). The effects of various factors on the proposed method including time, temperature, pH, order of the addition of various reagents and the concentration of AgNPs were investigated. Under the optimal conditions, a good linear relationship between the enhanced emission intensity of the Tb(III)-phen-AgNPs system and the PAN concentration was observed in the range of (10 – 1000) × 10−8 M. The limit of detection (LOD) and the limit of quantitation (LOQ) were 7.2 × 10−8 and 24.2 × 10−8 M, respectively. Also, the interferences of some common interfering species on the fluorescence intensity of the system were investigated. This simple and sensitive method was successfully applied for the determination of PAN in spiked human plasma samples and in its capsule formulation. The analytical recoveries were in the range of 88.54 – 101.33 and 90.07 – 98.85%, respectively.