Analytical Sciences

Abstract − Analytical Sciences, 36(11), 1317 (2020).

BODIPY-based Fluorescent Probe for the Detection of Cysteine in Living Cells
Nannan WANG, Xin JI, Han WANG, Xianhui WANG, Yanfang TAO, Weili ZHAO, and Jian ZHANG
Key Laboratory for Special Functional Materials of Ministry of Education, School of Materials Science and Engineering, Henan University, Kaifeng, 475004, P. R. China
Cysteine (Cys), as one of the important amino acids, plays a vital role in various physiological and pathological processes. Hence, it is meaningful to develop a convenient and sensitive detection method. Herein, a novel BODIPY-based fluorescent probe (BDP-DM) was developed, which had a higher selectivity for Cys than other amino acids, including homocysteine (Hcy) and glutathione (GSH). Ultimately, we concluded that the BDP-DM probe could be used to successfully detected intracellular Cys in living HeLa cells.