Analytical Sciences

Abstract − Analytical Sciences, 35(7), 803 (2019).

Development of Tube Radial Distribution Chromatography Based on Phase-Separation Multiphase Flow Created via Pressure Loss
Susumu WADA,* Kazuhiko TSUKAGOSHI,* Katsumi TSUCHIYA,* Ken HIROTA,* Kenichi YAMASHITA,** and Masaharu MURATA***
*Applied Chemistry, Graduate School of Science and Engineering, Doshisha University, Kyotanabe, Kyoto 610-0321, Japan
**Advanced Manufacturing Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 807-1 Shuku-machi, Tosu, Saga 841-0052, Japan
***Department of Advanced Medical Initiatives, Faculty of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi, Fukuoka 812-8582, Japan
A tube radial distribution chromatography (TRDC) method based on phase-separated multiphase flow created through phase transformation via temperature change has been developed. These systems typically required a temperature-controlling device containing a water bath and a stirrer. Herein, we proposed a novel TRDC system without a cooling device, where the phase transformation was achieved via pressure loss in a capillary tube of 50 μm inner diameter and 550 cm length. Model analytes were successfully separated with the developed TRDC system, which provided a simplified platform and helped to clarify the operating principle of TRDC based on phase transformation in a capillary tube.