Analytical Sciences

Abstract − Analytical Sciences, 35(4), 385 (2019).

A Highly Efficient Chemiluminescence System Based on an Enhancing Effect of Ag Nanoclusters/Graphene Quantum Dots Mixture for Ultrasensitive Detection of Rabeprazole
Ashraf YOUSEFZADEH,* Jafar ABOLHASANI,* Javad HASSANZADEH,** and Mohammad Hossein SOMI***
*Department of Chemistry, Tabriz Branch, Islamic Azad University, Tabriz, Iran
**Research Laboratory of Advanced Water and Wastewater Treatment Processes, Department of Applied Chemistry, Faculty of Chemistry, University of Tabriz, 51666-16471 Tabriz, Iran
***Liver and Gastrointestinal Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
Herein, an efficient chemiluminescence (CL) reaction with a high emission intensity is reported based on a synergistic improving effect of silver nanoclusters (AgNCs) and graphene quantum dots (GQDs). First, the syntheses of AgNCs and GQDs were simply performed by the chemical reducing of AgNO3 and a thermal treatment of glucose, respectively. After the characterization steps, the beneficial behavior of the prepared nanomaterial was investigated in CL systems. The oxidation reaction of KMnO4-rhodamine B produced weak CL emission. However, the presence of AgNCs and GQDs led to a synergetic enhancing effect, and thus higher emission was obtained. A possible mechanism was investigated for this effect using absorption and fluorescence experiments. Furthermore, rabeprazole showed a relatively selective enhancing impact on the CL emission. The CL intensity was linearly increased in the rabeprazole concentration range of 4 – 133 ng mL−1 with a detection limit (3Sb/m) of 1.1 ng mL−1. The developed CL method was utilized for the measurement of Rbp in biological samples with acceptable precision and accuracy.