Analytical Sciences

Abstract − Analytical Sciences, 35(10), 1135 (2019).

Water-soluble Hemin-mPEG-enhanced Luminol Chemiluminescence for Sensitive Detection of Hydrogen Peroxide and Glucose
Ze WANG, Bin DONG, Guodong FENG, Hongyan SHAN, Yanfu HUAN, and Qiang FEI
Department of Analytical Chemistry, College of Chemistry, Jilin University, Jiefang 2519, Changchun 130012, P. R. China
In the present study, we synthesized a water-soluble substance (Hemin-mPEG) at room temperature by using hemin and poly(ethylene glycol) methyl ether (mPEG). It was found that the Hemin-mPEG maintained the excellent catalytic activity inherited from hemin, and was first used to catalyze a luminol–H2O2 chemiluminescence (CL) system to generate an intense and slow CL signal. The results of a mechanism research showed that the presence of Hemin-mPEG could promote the production of oxygen-relative radicals from H2O2 and dissolved oxygen in solution. Based on this mechanism, an ultra-sensitive, cheap and simply practical sensor for detecting glucose and H2O2 was developed. Under the most optimal experimental conditions, H2O2 and glucose detection results exhibited a good linear range from 0.002 to 3 μM and from 0.02 to 4 μM, respectively, and the detection limits were 1.8 and 10 nM, respectively. This approach has been successfully used to detect glucose in actual biological samples, and achieved good results.