Analytical Sciences

Dual-channel Microchip Electrophoresis with Amperometric Detection System for Rapid Analysis of Cefoperazone and Sulbactam

Yan TAN,* Qianhui SONG,* Wenfang LIU,* Ming LI,** Jian XIAO,*** and Chuanpin CHEN*

*Xiangya School of Pharmaceutical Sciences, Central South University, Changsha, Hunan 410013, China
**School of Environmental Science and Engineering, Yangzhou University, Yangzhou, Jiangsu 225127, China
***Department of Pharmacy, Xiangya Hospital, Central South University, Changsha, Hunan 410013, China

A dual-channel microchip electrophoresis (ME) with in-channel amperometric detection was developed for cefoperazone and sulbactam determination simultaneously. In this study, a microelectrode detector was made of gold nanoparticles (GNPs) modified indium tin oxide (ITO)-coated poly-ethylene terephthalate (PET) film. The parameters including detection potential applied on working electrode, buffer concentration and pH value were optimized to improve the detection sensitivity and separation efficiency of cefoperazone and sulbactam. Under the optimal conditions, sensitive detection of cefoperazone and sulbactam was obtained with limits of detection (LODs) (S/N = 3) of 0.52 and 0.75 μg/mL, respectively. The plasma sample, which was from a patient with a brain injury taking Sulperazone, was successfully detected with a simple sample pretreatment process by dual-channel ME amperometric detection. This rapid and sensitive method possesses practical potential in clinical applications, and could provide a guidance for clinical rational drug use.

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