Analytical Sciences

Abstract − Analytical Sciences, 34(9), 997 (2018).

Visualization of Asparaptine in Asparagus (Asparagus officinalis) Using MALDI-IMS
Kohei MIYOSHI, Yosuke ENOMOTO, Eiichiro FUKUSAKI, and Shuichi SHIMMA
Department of Biotechnology, Graduate School of Engineering, The University of Osaka, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan
Asparaptine, an inhibitor of angiotensin-converting enzyme (ACE), is a newly discovered compound in asparagus. Asparaptine is a conjugate of arginine and asparagusic acid; this compound is suggested to be effective for preventing high blood pressure. For this reason, asparaptine has attracted remarkable attention in recent years, and it was therefore necessary to carry out further research. No studies to date have investigated the localization of asparaptine in asparagus. In this study, the localization of asparaptine in asparagus was clarified using matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS), a powerful method to visualize molecules.