Analytical Sciences

Abstract − Analytical Sciences, 33(2), 133 (2017).

A Label-free and Turn-on Fluorescence Strategy for Kanamycin Detection Based on the NMM/G-quadruplex Structure
Hualin YANG,* Qinghua WU,** Dongxiao SU,* Yun WANG,* Li LI,* and Xingping ZHANG*
*Jingchu Food Research and Development Center, College of Life Science, Yangtze University, Jingzhou, Hubei 434025, China
**Department of Chemistry, Faculty of Science, University of Hradec Kralove, Hradec Kralove, Czech Republic
In the present work, a simple design of a “label-free” fluorescence method for kanamycin was developed and explored based on a specific DNA aptamer as a recognizer and a NMM/G-quadruplex DNA system as a reporter. The titration experiment showed a linear detection range of 0.5 to 100 nM with a low detection limit of 0.5 nM. It also showed excellent selectivity in a selectivity experiment. It was then successfully employed to detect kanamycin in milk with a excellent reliability.