Analytical Sciences

Abstract − Analytical Sciences, 32(2), 153 (2016).

Development of a High-Sensitivity Quantitation Method for Arginine Vasopressin by High-Performance Liquid Chromatography Tandem Mass Spectrometry, and Comparison with Quantitative Values by Radioimmunoassay
Yasuko TSUKAZAKI,*1 Naoto SENDA,*1,*2 Kinya KUBO,*2 Shigeru YAMADA,*3 Hiroyuki KUGOH,*2,*4 Yasuhiro KAZUKI,*2,*4 and Mitsuo OSHIMURA*2
*1 Analytical Technology Research Department, LSI Medience Corp., 2-1-6 Sengen, Tsukuba, Ibaraki 305-0047, Japan
*2 Chromosome Engineering Research Center, Tottori University, 86 Nishi-cho, Yonago, Tottori 683-8503, Japan
*3 K. K. AB SCIEX, 4-7-35 Kitashinagawa, Shinagawa, Tokyo 140-0001, Japan
*4 Department of Biomedical Science, Institute of Regenerative Medicine and Biofunction, Graduate School of Medical Science, Tottori University, 86 Nishi-cho, Yonago, Tottori 683-8503, Japan
Human plasma arginine vasopressin (AVP) levels serve as a clinically relevant marker of diabetes and related syndromes. We developed a highly sensitive method for measuring human plasma AVP using high-performance liquid chromatography tandem mass spectrometry. AVP was extracted from human plasma using a weak-cation solid-phase extraction plate, and separated on a wide-bore octadecyl reverse-phase column. AVP was quantified in ion-transition experiments utilizing a product ion (m/z 328.3) derived from its parent ion (m/z 542.8). The sensitivity was enhanced using 0.02% dichloromethane as a mobile-phase additive. The lower limit of quantitation was 0.200 pmol/L. The extraction recovery ranged from 70.2 ± 7.2 to 73.3 ± 6.2% (mean ± SD), and the matrix effect ranged from 1.1 – 1.9%. Quality-testing samples revealed interday/intraday accuracy and precision ranging over 0.9 – 3% and –0.3 – 2%, respectively, which included the endogenous baseline. Our results correlated well with radioimmunoassay results using 22 human volunteer plasma samples.