Analytical Sciences

Abstract − Analytical Sciences, 32(12), 1295 (2016).

Oxime Based Selective Fluorescent Sensor for Arsenate Ion in a Greener Way with Bio-Imaging Application
Malay DOLAI,* Rabiul ALAM,* Atul KATARKAR,** Keya CHAUDHURI,** and Mahammad ALI*
*Department of Chemistry, Jadavpur University, Kolkata 700032, India
**Molecular & Human Genetics Division, CSIR-Indian Institute of Chemical Biology, 4, Raja S. C. Mallick Road, Kolkata 700032, India
A newly designed oxime based probe, 2,4-dihydroxyacetophenone-oxime (DHAO), was found to recognize H2AsO4 selectively with ∼3 fold "turn-on" fluorescence enhancement and LOD of 29 μM, K = (2.10 ± 0.54) × 104 M–1 in purely aqueous medium. The structures of the DHAO and its adduct with H2AsO4 were optimized by DFT calculations. Intracellular imaging of As(V) in HepG2 cells demonstrate the possibilities of in vitro/in vivo applications for selective monitoring of such species.