Analytical Sciences

Abstract − Analytical Sciences, 32(1), 113 (2016).

Patterned Co-culture of Live Cells on a Microchip by Photocrosslinking with Benzophenone
Kiichi SATO,* Sayaka KIKUCHI,* Eri YOSHIDA,** Reina ISHII,** Naoki SASAKI,** Kin-ichi TSUNODA,* and Kae SATO**
*Department of Chemistry and Chemical Biology, School of Science and Technology, Gunma University, Kiryu, Gunma 376-8515, Japan
**Department of Chemical and Biological Sciences, Faculty of Science, Japan Women’s University, Bunkyo, Tokyo 112-8681, Japan
The patterned coculture of different types of living cells in a microfluidic device is crucial for the analysis of cellular interactions and cell-cell communication. In the present study, cell patterning was achieved by photocrosslinking benzophenone derivatives in a microfluidic channel. Optimization of UV irradiation conditions enabled successful fixation of live cells. In addition, patterning and co-culture of non-adherent K562 cells and adherent RF-6A cells was achieved by successive rounds of patterning. The present approach is expected to be useful for the development of in vitro methods for studying cell signaling.