Analytical Sciences

Abstract − Analytical Sciences, 31(3), 139 (2015).

High-Contrast Fluorescence Imaging Based on the Polarization Dependence of the Fluorescence Enhancement Using an Optical Interference Mirror Slide
Mitsuru YASUDA and Takuo AKIMOTO
School of Bioscience and Biotechnology, Tokyo University of Technology, 1404-1 Katakura, Hachioji, Tokyo 192-0982, Japan
High-contrast fluorescence imaging using an optical interference mirror (OIM) slide that enhances the fluorescence from a fluorophore located on top of the OIM surface is reported. To enhance the fluorescence and reduce the background light of the OIM, transverse-electric-polarized excitation light was used as incident light, and the transverse-magnetic-polarized fluorescence signal was detected. As a result, an approximate 100-fold improvement in the signal-to-noise ratio was achieved through a 13-fold enhancement of the fluorescence signal and an 8-fold reduction of the background light.