Analytical Sciences

Abstract − Analytical Sciences, 31(12), 1211 (2015).

Fluorescence Probing Live Single-cell Mass Spectrometry for Direct Analysis of Organelle Metabolism
Tsuyoshi ESAKI and Tsutomu MASUJIMA
Quantitative Biology Center (QBiC), RIKEN, 6-2-3 Furuedai, Suita, Osaka 565-0874, Japan
Mitochondria in a live HepG2 cell were visualized with a fluorescent probe to specify their location and state in a living cell. Then, mitochondria were selectively captured with a nanospray tip under fluorescence microscope, and thousands of small molecular peaks were revealed and unique steroids specific to mitochondria were also found. This fluorescence imaging combined with live single-cell mass spectrometry opens the door to the analysis of site- and state-specific molecular detection to elucidate precise molecular mechanisms at the single-cell and organelle level.