Analytical Sciences

Abstract − Analytical Sciences, 30(4), 519 (2014).

Facile and Effective Pretreatment Using Stop and Go Extraction Tips for LC-MS/MS Analysis of Trace Amounts of DNA Adducts
Hiroya MURAKAMI,* Rieko KAWAMURA,* Takayoshi SAKAKIBARA,* Yukihiro ESAKA,* Yasushi ISHIHAMA,** and Bunji UNO*
*Gifu Pharmaceutical University, Daigaku-nishi, Gifu 501-1196, Japan
**Graduate School of Pharmaceutical Sciences, Kyoto University, Yoshida Shimoadachi-cho, Sakyo, Kyoto 606-8501, Japan
The preparation and application of a stop and go extraction tip (StageTip) used for pre-purification of sample solutions for LC-MS/MS analysis of DNA adducts was simplified and improved to increase throughput while maintaining high adduct selectivity. It was demonstrated that the StageTip composed of two sheets of a poly(styrene-divinylbenzene) copolymer disk could be easily prepared and proved useful for selective extraction of trace amounts of DNA adducts from a sample solution containing a great quantity of normal deoxynucleosides.