Analytical Sciences

Abstract − Analytical Sciences, 30(4), 457 (2014).

Sensitive Sulfide Sensor with a Trypsin-stabilized Gold Nanocluster
Jun FAN, Ruiping LI, Pingping XU, Junwei DI, Yifeng TU, and Jilin YAN
The Key Lab of Health Chemistry and Molecular Diagnosis of Suzhou College of Chemistry, Chemical Engineering and Materials Science, Soochow University, 199 Ren’ai Road, Industrial Park, Suzhou 215123, P. R. China
In this work, we synthesized a trypsin-stabilized fluorescent gold nanocluster. It was found that sulfide interacted with the nanocluster, which could result in significant fluorescence quenching. With this quenching effect, a fluorescence sulfide sensor was developed. This sensor responded linearly to sulfide in the range of 50 nM to 8 μM, and was capable of detecting sulfide as low as 5.5 nM. This provided a facile and sensitive scheme for sulfide analysis; the mechanism of the sensor was also provided. The sensor was then tested for real sample analysis, and good recoveries were obtained. Furthermore, persulfate was found to be effective to remove the quenching of sulfide, and this interaction was adopted for an indirect analysis of persulfate.