Analytical Sciences

Abstract − Analytical Sciences, 30(3), 383 (2014).

Quantitative Analysis of D-(+)-Glucose in Fruit Juices Using Diffusion Ordered-1H Nuclear Magnetic Resonance Spectroscopy
Ruge CAO,* Fusae KOMURA,* Airi NONAKA,* Takeshi KATO,** Junji FUKUMASHI,** and Toshiro MATSUI*
* Division of Bioresources and Biosciences, Faculty of Agriculture, Graduate School of Kyushu University, 6-10-1 Hakozaki, Fukuoka 812-8581, Japan
**Tama Laboratory, Japan Food Research Laboratories, 6-11-10 Nagayama, Tama, Tokyo 206-0025, Japan
This study works on D-(+)-glucose quantitative analysis using diffusion ordered-quantitative 1H nuclear magnetic resonance spectroscopy (DOSY-qNMR), by which an analyte could be distinguished from interferences based upon a characteristic diffusion coefficient (D) in gradient magnetic fields. The D value of D-(+)-glucose in deuterium oxide at 30°C was 5.6 × 10−10 m2/s at a field gradient pulse of between 5.0 × 10−2 and 3.0 × 10−1 T/m, distinguished from fructose, sucrose and starch. Good linearity (r2 = 0.9998) was obtained between D-(+)-glucose (0.5 – 20.0 g/L) and the ratio of the resonance area of α-C1 proton (5.21 ppm) in D-(+)-glucose to that of the β-C1 proton (5.25 ppm) in D-glucuronic acid (50.0 g/L) as an internal standard. The DOSY-qNMR method was successfully applied to quantify D-(+)-glucose in orange juice (18.3 ± 1.0 g/L), apple juice (26.3 ± 0.4 g/L) and grape juice (45.6 ± 0.6 g/L); the values agreed well with a conventional F-kit glucose method.