Analytical Sciences

Abstract − Analytical Sciences, 30(12), 1103 (2014).

A Facile Preparation of Glass-supported Lipid Bilayers for Analyzing Molecular Dynamics
Yuma ITO,*,** Kumiko SAKATA-SOGAWA,*,** and Makio TOKUNAGA*,**
*Department of Biological Information, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Nagatsuta-cho, Midori, Yokohama, Kanagawa 226-8501, Japan
**Center for Integrative Medical Sciences, RIKEN, Suehiro-cho, Tsurumi, Yokohama, Kanagawa 230-0045, Japan
Many research programs focus on the molecular dynamics of living cells. This research requires cells to be adhered to a substrate while retaining the innate motility of their surface molecules. Lipid bilayer-based systems fulfill this requirement, although current methods are complicated and their utility is limited. We developed a simple and rapid method for reproducible preparation of homogeneous glass-supported lipid bilayers. Our method provides a facile means for bioimaging and analysis of molecular dynamics in living cells.