Analytical Sciences

Abstract − Analytical Sciences, 29(8), 811 (2013).

Simple Preparation of Green Fluorescent Protein Conjugated with β-Cyclodextrin in a Site Specific Manner
Miho SUZUKI,* Yoshihiro ISHIMARU,* Ayumu SAITO,** and Koichi NISHIGAKI*
*Department of Functional Materials and Science, Graduate School of Science and Engineering, Saitama University, 255 Shimo-okubo, Sakura, Saitama, Saitama 338-8570, Japan
**Human Genome Center, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato, Tokyo 108-8639, Japan
We have site-directedly linked a green fluorescent protein (GFP) variant and a β-cyclodextrin (β-CD) with a simple method to develop a basic complex for sophisticated supramolecules. We have confirmed β-CD grafting on GFP with several methods including matrix-assisted laser desorption/ionization linear time-of-flight mass spectrometry (MALDI-TOF MS) without protease digestion and characterized the complex as well. In consideration of the resulting properties, the product we plainly and efficiently obtained could have applications related to sensing devices and drug delivery systems.