Analytical Sciences

Measurement of Pore Diffusivity in Separation Media for High Performance Liquid Chromatography

Kanji MIYABE,* Yuko MATSUMOTO,** Nobuho ANDO,** and Yumi TERATANI**

*Department of Chemistry, Faculty of Science, Rikkyo University, 3-34-1 Nishi-Ikebukuro, Toshima, Tokyo 171-8501, Japan
**Faculty of Engineering, University of Toyama, 3190 Gofuku, Toyama 930-8555, Japan

It was demonstrated that the peak parking-moment analysis (PP-MA) method could be used as an alternative for the measurement of pore diffusivity (D_p) in porous packing materials for HPLC. The value of D_p of uracil was measured by the PP-MA method under non-retained conditions in the RPLC systems using C₁₈-silica gel particles and C₁₈-silica monolith as the stationary phase and 70 vol% methanol as the mobile phase. The value of D_p was compared with that derived by the pulse response-moment analysis (PR-MA) method under the same conditions. The D_p values of uracil derived by the PP-MA and PR-MA methods were almost in agreement with each other. Because the PP-MA and PR-MA methods have complementary characteristics as experimental methods for studying mass transfer kinetics, it is expected that the combination of the two methods would lead to an effective strategy for the measurement of D_p in porous separation media for HPLC.

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