Analytical Sciences

Abstract − Analytical Sciences, 29(1), 41 (2013).

Bioluminescence Inhibition of Bacterial Luciferase by Aliphatic Alcohol, Amine and Carboxylic Acid: Inhibition Potency and Mechanism
Shinya YAMASAKI,* Shuto YAMADA,** and Kô TAKEHARA*
*Department of Chemistry, Faculty of Sciences, Kyushu University, 6-10-1 Hakozaki, Fukuoka 812-8581, Japan
**Center for Research and Advancement in Higher Education, Kyushu University, 744, Motooka, Fukuoka 819-0395, Japan
The inhibitory effects of hydrophobic molecules on the bacterial luciferase, BL, luminescence reaction were analyzed using an electrochemically-controlled BL luminescence system. The inhibition potency of alkyl amines, CnNH2, and fatty acids, CmCOOH (m = n – 1), on the BL reaction increased with an increase in the alkyl chain-length of these aliphatic compounds. CmCOOH showed lower inhibition potency than CnNH2 and alkyl alcohols, CnOH, data for which have been previously reported. To make clear the inhibition mechanisms of the aliphatic compounds on the BL reaction, the initial rate of the BL reaction was measured and analyzed using the Dixon plot and Cornish–Bowden plot. The C12OH inhibited the BL reaction in competition with the substrate C11CHO, while C12NH2 and C11COOH inhibited in an uncompetitive manner with the C11CHO. These results suggest that the alkyl chain-length and the terminal unit of the aliphatic compound determine the inhibition potency and the inhibition mechanism, respectively.