Analytical Sciences

Abstract − Analytical Sciences, 29(1), 21 (2013).

Chemiluminescence Detection of Glucose Using Ce(IV) Oxidation in a Batch System
Megumi NAGAI,* Wakayo MIYAHARA,* Honami SAGAWA,* Shigeo YAMAZAKI,* and Keiitsu SAITO**
*Separation Science Division, Faculty of Science, Okayama University of Science, 1-1 Ridaicho, Okayama 700-0005, Japan
**Division of Natural Environment and Chemistry, Faculty of Human Development, Kobe University, 3-11 Tsurukabuto, Nada, Kobe 657-8501, Japan
A chemiluminescence method for the determination of glucose (GC) using a batch system made in our laboratory is proposed. The method is based on measures of the chemiluminesence intensity from a batch of sample GC solutions following the injection of an (NH4)2[Ce(NO3)6]–[Ru(bpy)3]Cl2 solution. The effect of the Ru complex concentration on chemiluminescence was studied. The detection limit was 0.5 × 10−8 M, and the linear calibration curve reached 10−7 M. The Ru complex was selected on the basis of its desirable performance compared to those of other photosensitizers. However, flow-injection analysis (FIA) using the Ce-Ru complex reagent was not applicable for the detection of GC.